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Count Butler is the main antagonist of the third movie. He claims to be a genius zoologist, but in reality, he is actually a poacher searching for the Crowning Treasure horns on Crown Island. He is assisted by Hotdog and Heaby. (Source: One Piece wiki)

Karasuke is a character from the third One Piece Movie. He is a talking crow that is close friends with Mobambi and is the only other animal, besides Bald Parrot, on the island that can talk to humans. He helps Mobambi help Chopper when the other animals abandon Chopper. (Source: One Piece wiki)

Manda is the personal summon of Orochimaru. Manda was the largest snake in the world, before being recently out-sized by his clone, Manda II. He easily towers over buildings, trees, and large rock formations. Due to his length, he is even larger than Gamabunta and Katsuyu, easily coiling around Katsuyu and having more length behind him, and he could open his jaws wide enough to swallow Gamabunta whole. He is colored purple, and has black rings running down intervals on his body. He has massive fangs, though they are arranged in a way similar to that of a constrictor snake instead of a viper or other venomous snake species, presumably carrying venom in them from the way they were designed, and green eyes. He also has four horn-like protrusions on his head.

A faithful pet to Princess Megan. He was given to her as a baby when she was 7 years old. Megan is the only person capable of understanding Mango. He is bright orange, with orange eyes, green hair and two small wings on his back. (Wikipedia)

A Goi (non-magician) and was a former inhabitant of the 5th Level Authorization District of Magnostadt. She was rescued by Titus Alexius whom she forged a special bond with.

Sakuya is the only human living in the Scarlet Devil Mansion, yet she is also the head maid. The official fanbook suggests she had been a vampire hunter before she began working in the mansion, but she was defeated by Remilia Scarlet who pitied her and gave Sakuya her current name. Though she looks about sixteen like the other girls, Sakuya has the ability to slow, stop and possibly reverse time. She seems to have been serving Remilia for a long time and may be far older then she looks. Socially, she interacts mainly with the others in the Scarlet Devil Mansion. Her talents are cooking, knife throwing and time manipulation. The latter two abilities characterize her appearances in-game, and led to frequent parodies in reference to Dio Brando of JoJo's Bizarre Adventure, who also controls time and throws knives. This reference goes so far as Sakuya using some of his poses in a game. She is often associated with dogs by other characters because she is serving as a demon's "faithful guard dog" even though she is a full blood human. Due to the inconsistent artwork depicting her bust between games, d�jinshi often make fun of her by indicating that she pads her breasts. (Source: Wikipedia)