Nan was a villager in a small village in the demilitarized zone of the Rice Field Country. The village had a small gold mine which became a target of bandits due to no government presiding over the area. Bandits led by Shiin attacked the village and killed a third of its citizens. Nan and her brother Ran survived. She looked after Fuki when the young girl’s parents died in the attack.
Tsubaki is Mizuki's lover. She claims that Mizuki used to be a sweet man, but ever since he got involved with Orochimaru, her relationship with Mizuki has faltered. When he escapes from prison she is able to help catch him. After she interrogates Mizuki about having a grudge against one who put his life on the line during Orochimaru's attack, Mizuki knocks out Tsubaki, who he apparently does not love anymore. After Mizuki was defeated, she mentioned to Iruka that she may have to face whatever consequences for her involvement with Mizuki. Source: Narutopedia
He is an anime-original character, originally the assistant of the machinery development department. Though he and his partners have come up with many ideas about how to make a virtual Embryo or catch the Embryo through X-Eggs, mostly resulted in failures and despised by Kazuomi.
Mana is a childhood friend of Atem and Mahad and studies magic under Mahad as his apprentice. She is a light-hearted, open, playful and caring girl, who shares a deep bond between her master and Atem. Mana is a young magician in training at the Dawn of the Duel arc's beginning who serves the priest Mahad. In the Manga she first appeared before Mahad after hiding in the pots outside of the palace. Mahad sensed her there and told her to come out. After popping out of the pot, she exclaimed how she'd do her training later and Mahad responded by telling her she'd never become a sorcerer that way. After Mahad's death, she swears to become a great magician and eventually is able to summon her own Ka, the "Dark Magician Girl." Later when Yugi's gang discovers her, Yugi explains about the card game back home commenting on her reference to Dark Magician Girl. Mana later helps them to defeat Zorc. She is one of the only two people in the Millennium world who could see Yugi and his friends. A single panel in the manga shows that she inherited the Millennium Ring after Zorc was defeated.